Co-delivery of curcumin and si-STAT3 with a bioinspired tumor homing for polydopamine nanoparticles for synergistic osteosarcoma therapy

Abstract Purpose Owing to the complexity of cancer, a synergistic combination chemotherapy and gene therapy can be promising therapeutic strategy. This study aimed use stem cell membrane (SCM)-camouflaged polydopamine nanoparticles for simultaneous delivery curcumin (CUR) siRNA-targeting STAT3 (CPDA/ siSTAT3 @SCM NPs) osteosarcoma (OS). Methods Transmission electron microscopy, UV–Vis absorbance spectra, zeta potential, co-localization, Coomassie bright blue staining were used characterize CPDA/ NPs constructed by self-assembly method. Drug release, cellular uptake, proliferation, apoptosis, wound healing, transwell assays evaluated in vitro. The expression levels epithelial–mesenchymal transition (EMT)- apoptosis-related proteins measured western blotting. Furthermore, biodistribution, antitumor efficacy, biosafety an MG63 xenograft mouse model evaluated. Results successfully synthesized deliver CUR siRNA simultaneously, they showed osteosarcoma-targeting ability. it high uptake excellent effects suppressed OS migration, invasion, EMT progression, promoted apoptotic process. In tumor-bearing mice, treatment with effect no side major organs. Conclusion revealed that CPDA/siSTAT3@SCM target drug biomimetic multifunctional treat through chemo-gene combined therapy.